Adhatoda vasaca Leaf Section Cutting | How to Do Section Cutting & Microscopy of Leaf (ENGLISH)

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Leaf of Adhatoda vasica ( Vasaka ) is an important drug of Ayurveda, prescribed as an expectorant. For pharmacognosy and botany student it is very important to know the procedure for section cutting off any part o plant. To make a microscopic study we need to make a clear we need to make a clear section of Adhatoda vasica ( Vasaka ). For the preparation of section, we have followed following steps-

1- Collect fresh and clean leaves of Adhatoda vasica ( Vasaka )
2- Collect 04 watch glass which will be used in keeping the leaves of Adhatoda vasica ( Vasaka )
3- Place water, Safranin, Potato cube and again plain water in respective watch glass for Adhatoda vasica ( Vasaka )
4- Take a fresh leaf of Adhatoda vasica ( Vasaka ) and place it over the plain surface
5- now take a new and sharp razor blade to cut the leaf of Adhatoda vasica ( Vasaka )
6- Prepare the cubes of potato for further use for holding of Adhatoda vasica ( Vasaka )
7- Now cut the leaf of Adhatoda vasica ( Vasaka ) containing its midribs portion.
8- Transfer the cutted portion of Adhatoda vasica ( Vasaka ) in watch glass containing water
9- Now place that cut leaf of Adhatoda vasica ( Vasaka ) in between the opening of potato cubes.
10- Now cut the excess portion of leaves of Adhatoda vasica ( Vasaka ) through a razor blade.
11- Now move the blade through and forth direction
12- Place the leave of Adhatoda vasica ( Vasaka ) in watch glass containing water
13- Transfer the section of Adhatoda vasica ( Vasaka ) into a safranin contain watch glass
14- Now place that stained leaf of Adhatoda vasica ( Vasaka ) into watch glass containing plain water to remove excess color.
15- Place back the leaf into glass slide and add 2-3 drop of glycerin-water to prevent drying of Adhatoda vasica ( Vasaka )
16- Now add a coverslip over a slide containing leaf of Adhatoda vasica ( Vasaka )
17- Watch the leaf under a microscope and draw the structure.

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