Скачать или смотреть How changing the shape of an antioxidant protein affects its function, Storm Voyce-McCulloch

Peroxiredoxins are an enzyme family important in cellular processes due to their range of functions, namely the removal of reactive oxygen species. Altered activity of peroxiredoxins has been implicated in disease development such as cancer. Peroxiredoxins exist as two structures: one smaller, made of two parts, and the other a larger, ring-like structure. No methods are available to study the relationship between the different structures of peroxiredoxin and their activity in cells. Nanobodies are a new tool in the research of proteins and have previously been fluorescently tagged and used to study proteins in cells. This project has made progress in the development of nanobodies specific to peroxiredoxin, through introducing two mutations into the nanobody sequence. This sequence in future can be used to express mutant peroxiredoxin specific nanobodies, that can be fluorescently tagged, and used to study how peroxiredoxin structures relate to their activity in the cell.