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pUC8 is a plasmid vector that is used for cloning and expressing genes. It is a derivative of the pBR322 plasmid and the M13 filamentous phage. 

Features of pUC8

Size: pUC8 is 2,750 base pairs (bp) long. 

Copy number: pUC8 has a high copy number of 500–700 even before replication. 

Restriction sites: pUC8 has a cluster of restriction sites in the LacZ' gene. 

Lac promoter: pUC8 contains the beta-galactosidase gene under the control of the lac promoter. 

Blue-white color change: pUC8 can be used to identify recombinant colonies by their blue or white color on X-gal plates. 

Uses of pUC8

Cloning: pUC8 can be used to insert large foreign DNA fragments into the vector. 

Expression: pUC8 can be used as an inducible expression vector to express synthetic DNA, genomic DNA, or cDNA. 

Immunological screening: pUC8 can be used to screen cDNA or genomic DNA banks directly.