Скачать или смотреть How to Count Cells

This is a protocol on how to count cells using manual and automatic methods with Trypan Blue. Please modify as needed for your own lab spaces.
00:00 - 00:39 Introduction
00:39 - 04:57 Cell Count Introduction
04:57 - 07:46 Set up for Needed for Both Methods
0:7:46 - 16:11 Manual Counting
16:11 - 19:33 First Cell Math Problem
19:33 - 23:13 Automatic Counting
23:13 - 25:01 Cleaning Up Both Methods
25:01 - 32:01 Second Cell Math Problem

https://cellmanufacturingusa.org/
https://cellmanufacturingusa.org/
https://engineering.uga.edu/

How to Passage:
   • How To Passage Cells  
Steps shown up to 23:26 gets you to a cell suspension that can be sampled from. Be mindful that presoaking destination flasks with media may interfere with your ability to seed within the working volume (especially if your cell count is low). Do this with caution.