How to Make Sterilized Rye Grain Spawn for Growing Mushrooms

Rye Berries are my favorite grain to use to generate high quality grain spawn.

Soaking the rye grain overnight is recommended to germinate any endospores that can withstand sterilization temps. Once germinated, they will be susceptible to the sterilization cycle.

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I find it best to boil the water / turn off heat source and then dump the soaked/drained grains into the very hot water stirring periodically for 20-30 minutes. Drain and lay out of a towel for and let the steam evaporate the moisture.

Sterilization: 15 psi 2-2.5 hours

Shaking the jars after they have cooled down will help to distribute moisture.

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