Скачать или смотреть Molecular Video Lecture 04 (Nucleic Acid Quant, PCR, qPCR, MLPA)

Topics:
Review of techniques and their resolution 1:15
DNA quantiation 2:25
Spectrophotometry 3:07
Spectrophotometry of nucleic acids 4:13
Spectrophotometry of proteins 5:11
OD260/280 ratio 6:10
Measuring dsDNA by spot assay 9:00
dsDNA by fluorometry 10:43
Why DNA target amplification is needed 12:37
PCR 16:57
History of PCR 18:26
Components of PCR 19:37
Thermocycling 22:06
Taq polymerase 22:56
Thermocycling steps 24:00
Melting/denaturing 24:15
Annealing/PCR specificity 25:10
Extension 28:44
Exponential PCR dynamics 30:04
Single primer extension 32:57
Cycling 35:34
PCR phases 36:17
PCR dsDNA amplicon product 39:00
PCR readout 40:11
Agarose gel electrophoresis 40:26
Endpoint vs real-time PCR 43:35
Real-time PCR 47:54
How to make real-time PCR quantitative (qPCR) 52:05
Importance of internal reference controls in real-time qPCR 58:38
Affymetrix chromosomal microarray CNV normalization 1:03:40
qPCR design considerations 1:05:06
Taqman qPCR 1:06:56
Advantage of qPCR 1:10:19
Minimal residual disease (MRD) monitoring 1:12:00
Disadvantage of qPCR 1:13:00
Multiplex ligation probe amplification (MLPA) 1:14:20
Capillary electrophoresis 1:20:05