🦟 Detecting insecticide resistance in mosquitoes 🧬 (Lab @ Home Vlog)

This week Jenny tries out two protocols that several of our community members in the US have been developing, using Anopheles gambiae mosquitoes kindly provided by the CDC.

Protocol 1 uses PCR to identify which of the six species within the Anopheles gambiae species complex each mosquito belongs to.

Protocol 2 uses two PCRs to determine whether these mosquitoes possess knockdown resistance mutations (kdr-East or kdr-West) associated with insecticide resistance in East and West Africa, respectively.

Reagents and controls were provided by the CDC Malaria Research and Reference Reagent Resource Center (MR4) as part of BEI Resources. Protocols can be found at www.beiresources.org.
Use of trade names and commercial sources is for identification only and does not constitute endorsement by CDC or the U.S. Department of Health and Human Services.

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0:00 - Introduction
3:24 - Preparation of mosquitoes for DNA extraction
6:13 - HOTShot DNA extractions
8:33 - PCR for protocol 1 using HOTShot DNA extractions
10:57 - Loading the gel with PCR amplicons
12:25 - Results for PCR for protocol 1 using HOTShot DNA extractions
19:42 - Dipstick DNA extractions & repeat PCR for protocol 1
22:18 - Results for PCR for protocol 1 using Dipstick DNA extractions
23:55 - Kdr-East and kdr-West PCRs for protocol 2 using HOTShot DNA extractions
25:36 - Results for kdr-East and kdr-West PCRs
31:41 - Closing remarks