Скачать или смотреть Application of Variable Pathlength Technology for Rapid Determination of Dynamic Binding Capacity

An ever-increasing demand for biomolecule-based therapies is driving biopharmaceutical companies to accelerate process development activities to remain competitive in today’s industry. Within the downstream process development space, affinity chromatography is widely used for primary capture of monoclonal antibodies, Fc fusion proteins, etc. Optimization of this step requires determination of dynamic binding capacity (DBC) for target protein molecule and can be a lengthy process using traditional methods. KBI Biopharma has applied the use of variable pathlength technology for rapid generation of breakthrough curves and determination of DBC for capture resin selection. FlowVPE, a variable pathlength UV detector from Repligen, is integrated with the chromatographic system for real time generation of breakthrough curves, reducing the time and cost associated with the development of the primary capture step. This technology helps accelerate the overall process development timeline. Case studies are included.

About the speaker: Rashmi Bhangale, Ph.D - Downstream Process Development Scientist at KBI Biopharma. Rashmi received her Ph.D. in Chemical Engineering from the Institute of Chemical Technology in Mumbai, India. Her research work focused on understanding the mechanisms involved in chromatographic separations for a priori prediction of purification performance. Rashmi joined the Downstream Process Development team at KBI Biopharma in North Carolina in 2018. She is involved in the development and transfer of purification processes for a variety of molecules including monoclonal antibodies, Fc fusions, and enzymes.

Recorded on June 1, 2021 at 8am PDT | 11am EDT | 4pm GMT