Protein expression & purification techniques - overview of some common techniques & typical workflow

Here’s an overview of some of the common techniques used to express and purify proteins

- blog page: https://thebumblingbiochemist.com/let...  
- YouTube playlists 
- recombinant protein expression:    • Recombinant protein expression    
- protein purification:    • Protein chromatography    
- protein chromatography:    • Protein chromatography    
- molecular cloning:    • molecular cloning  

timestamps:  
0:00 intro
0:39 overview
4:25 chromatography overview
7:08 affinity chromatography
11:05 gravity flow vs FPLC (e.g. AKTA)
12:43 ion exchange chromatography
15:57 size exclusion chromatography (SEC) - aka gel filtration
18:41 molecular cloning
20:23 fusion partners
21:32 bacterial expression
24:03 codon optimization
25:54 insect cell expression (BEVS)
26:50 mammalian cell expression
27:10 cell-free protein expression
28:13 unnatural amino acid incorporation
29:05 harvesting & lysis
31:23 dialysis & desalting
33:14 centrifugal ultrafiltration (spin concentrators)
34:48 measuring concentration
34:50 SDS-PAGE
35:30 flash freezing

more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com