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FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors - a 2 minute Preview of the Experimental Protocol

Julia U. Sprenger, Ruwan K. Perera, Konrad R. Götz, Viacheslav O. Nikolaev
Georg August University Medical Center, Göttingen, Germany, Emmy Noether Group of the DFG, Department of Cardiology and Pneumology, European Heart Research Insitute Göttingen;

Förster resonance energy transfer (FRET) microscopy is a powerful technique for real-time monitoring of signaling events in live cells using various biosensors as reporters. Here we describe how to build a customized epifluorescence FRET imaging system from commercially available components and how to use it for FRET experiments.

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