Primary Endothelial Cells Isolation and Culturing from Canine | Protocol Preview

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Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins - a 2 minute Preview of the Experimental Protocol

Loes A. Oosterhoff, Hedwig S. Kruitwagen, Bart Spee, Frank G. van Steenbeek
Utrecht University, Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine;

Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.

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