Polymerase Chain Reaction (PCR): DNA Amplification

PCR is based on the mechanisms of DNA replication. First, the double-stranded DNA, which serves as the template in the reaction, is separated by heat. Next, a DNA polymerase synthesizes a new daughter strand on each single strand in one direction. By repeating these reactions continuously, a defined segment of DNA, the target, is amplified exponentially.

Further information can be found in the following AMBOSS article:

https://www.amboss.com/us/library#xid...

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