Culturing Primary Rat Inner Medullary Collecting Duct Cells l Protocol Preview

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Culturing Primary Rat Inner Medullary Collecting Duct Cells - a 2 minute Preview of the Experimental Protocol

Dörte Faust, Andrea Geelhaar, Beate Eisermann, Jenny Eichhorst, Burkhard Wiesner, Walter Rosenthal, Enno Klussmann
Max-Delbrück-Center for Molecular Medicine, Anchored Signalling; Leibniz Institute for Molecular Pharmacology (FMP),; Charité University Medicine Berlin,;

Arginine-vasopressin (AVP) controls fine-tuning of body water homeostasis through facilitating water reabsorption by renal principal cells. Here, we present a protocol for the cultivation of primary rat inner medullary collecting duct cells suitable for the elucidation of molecular mechanisms underlying AVP-mediated water reabsorption.

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