Ask a Scientist - What is GC-Bias?

What is GC-Bias in NGS, where does it come from, and how can it be reduced? This video provides an overview of GC-bias in next-generation sequencing (NGS), explaining how this metric is derived and the impact GC-bias has on sequencing data. It also explains how to interpret GC-bias distribution plots, and compares an example of theoretical, ideal coverage to examples of possible outcomes from varying data sets. Finally, the video offers tips to decrease GC-bias and increase coverage uniformity in NGS, including hybridization-based target enrichment workflows.

Presented by Drew Cheney, Field Applications Scientist at Roche Sequencing and Life Sciences

MC-US-08182