Скачать или смотреть Simultaneous Cryosectioning of Brains | Protocol Preview

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Simultaneous Cryosectioning of Multiple Rodent Brains - a 2 minute Preview of the Experimental Protocol

Tabitha R.F. Green, J. Bryce Ortiz, Jordan L. Harrison, Jonathan Lifshitz, Rachel K. Rowe
University of Arizona College of Medicine, Department of Child Health; University of Bath, Department of Biological Sciences; BARROW Neurological Institute at Phoenix Children's Hospital,; Phoenix Veteran Affairs Healthcare System,; University of Arizona College of Medicine, Department of Basic Medical Sciences;

Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.

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