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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies - a 2 minute Preview of the Experimental Protocol

Todd C. Lorenz
University of California, Los Angeles , Microbiology, Immunology, and Molecular Genetics;

PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.

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