Скачать или смотреть Lecture On Enzyme Kinetics Explained | Michaelis-Menten, Lineweaver-Burk, KM & Vmax | MBBS

This detailed lecture, part of the Santiniketan Medical College MBBS (2025-2026 Batch) series, breaks down the complex topic of Enzyme Kinetics. We cover the core principles of reaction velocity, the defining Michaelis-Menten model, the practical use of the Lineweaver-Burk plot, and the different types of enzyme inhibition.

This video is an essential guide for biochemistry exams, understanding metabolic pathways, and grasping the action of pharmaceutical drugs that target enzymes.
Lecture Highlights & Timestamps for Easy Navigation

[00:00] Factors Affecting Enzyme Reaction Rate:

Effect of Enzyme Concentration (Direct Proportionality).

Effect of Temperature (Optimum Temperature and Denaturation).

Effect of pH (Optimum pH examples: Pepsin, Salivary Amylase, Alkaline Phosphatase).

[07:15] Effect of Substrate Concentration on Reaction Velocity (V0) and reaching Vmax (Maximum Velocity).

[11:04] The Michaelis-Menten Equation and its components (V0, Vmax, [S], KM).

[16:46] Understanding the Michaelis Constant (KM): Historical Context (Michaelis-Menten vs. Briggs-Haldane) and its true definition.

[26:03] How to Determine the KM Value: Amount of substrate required for half the maximum velocity (Vmax/2).

[28:32] Steady State Kinetics: Understanding the concept where the concentration of the Enzyme-Substrate (ES) complex remains constant.

[34:48] The Lineweaver-Burk Plot (Double Reciprocal Plot): Why a linear graph is preferred for calculations.

[37:35] Clinical Correlation: Comparing Hexokinase and Glucokinase – The significance of their different KM values for glucose metabolism in different tissues (e.g., Liver vs. Brain).

[41:53] Introduction to Enzyme Inhibition: Classification (Reversible, Irreversible, etc.).

[46:44] Detailed Analysis of Competitive Inhibition: Mechanism, effect on KM (KM increases), and effect on Vmax (Vmax remains same).


SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: BIOCHEMISTRY by Dr. Sandip Ghosh
(MBBS Batch: 2025-26)
Conducted on: 01-12-25