HPLC PROBLEMS | HPLC TROUBLESHOOTING

HPLC PROBLEMS | HPLC TROUBLESHOOTING ‎@voiceofkayani5419 

This video based on HPLC problems and troubleshooting.
hope it will help you in HPLC analysis.
thank you for watching.

Your quires;
HPLC
HPLC problems
HPLC troubleshooting
Peak Tailing
1- high concentration sample used
2- mobile phase pH incorrectly maintained
3- incorrect sample preparation
4- incompatibility sample last dilution
5- overland injections on HPLC column

Resolution Problem
1- required mobile phase tuning
2-in correct sample preparation

unstable baseline;
1- used AR Hplc grade solvent
2- contamination in system
3- incorrect pH of Mobile phase

RT SHIFT;
1-incorrect pH of mobile phase
2-Mobile phase composition disturbed

#hplc #troubleshooting #voiceofkayani

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