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HPLC Vcauum Mobile phase Degassing
When different liquids mix in HPLC analysis, bubbles forming can cause problems. To avoid this, we removed the bubbles by degassing the liquid used in the analysis.

HPLC Degassing Techniques for Laboratory
Commonly used degassing practices for the HPLC mobile phase are:

Helium purging
Vacuum degassing
Sonication system

Vacuum degassing can take out more than 60% of the air. One way is to use a special filter while pulling the liquid through it with a vacuum. Many commercial systems have a way to do this while the liquid is still in the machine. The special tubing inside removes the air but keeps the liquid inside.

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