Скачать или смотреть Probing mRNA Kinetics In Space & Time: E coli By Two-Color Single-Molecule FISH l Protocol Preview

Watch the Full Video at https://www.jove.com/v/61520/probing-....

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization - a 2 minute Preview of the Experimental Protocol

Sangjin Kim, Kavya Vaidya
University of Illinois at Urbana-Champaign, Department of Physics; University of Illinois at Urbana-Champaign, Center for the Physics of Living Cells; University of Illinois at Urbana-Champaign, Carl R. Woese Institute for Genomic Biology; University of Illinois at Urbana-Champaign, Center for Biophysics and Quantitative Biology;

This protocol describes an application of single-molecule fluorescence in situ hybridization (smFISH) to measure the in vivo kinetics of mRNA synthesis and degradation.

Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel:    / jovejournalofvisualizedexperiments