Скачать или смотреть Sucrose Density Gradient Experiment

In this condensed ~90 second video we are using a Sucrose Density Gradient of different layers (5, 10, 15 and 20%). On top of this gradient a smoothie is layered on the surface. The tube is than placed inside the centrifuge for a total of 15 minutes. By speeding up the process we can a quick grasp on how the particles in the smoothy move and get concentrated in the different layers and create "banding" in the Sucrose Density Gradient under 2500 g / RCF.

What would you like to see separated inside a centrifuge.

-------- Work in progress --------
These video's and project are a work in progress, there are still many adjustments that need to be made to the centrifuge to create an even more optimised recording environment. In regards to the channel format I'm looking into what would be the best way to present these videos, talking and informing about what is happening to the sample from all different (science) perspectives to be able to give the audience a better understanding. Next to that

I'm also looking for help from the (science) community for the following:
Supporting the project with test samples and / or suggestions on what to centrifuge and/or ways to do this accordingly to simulate existing lab experiments.
Recommendations, tips and or tricks on how to get better visual results.
Recommendations, tips and or tricks on how to analyse the samples.
Leads on a larger and higher RCF centrifuge to expand the capabilities of the project.

If you like to help this project out? I would be more than pleased to hear from you.

00:00 Intro
00:03 Centrifuge ramping up towards 2500g / RCF @4000RPM.
00:10 The smoothie is layered on top but already settled on top of the 15% sucrose layer
00:20 Cells are "migrating" through the 15% sucrose layer.
00:30 Cells are slowed down at the top of the 20% sucrose layer.
00:35 We can clearly see red "banding" occuring on top of the 15% sucrose layer.
00:40 Cells start to "migrate" through the 20% sucrose layer creating a pellet at the bottom.
01:00 We can now see the red "banding" on top of the 15% sucrose and cells being pelleted at the bottom.
01:27 Centrifuge Comes to a full stop.