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Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila - a 2 minute Preview of the Experimental Protocol

Eugenia Villa-Cuesta, David M. Rand
Adelphi University, Biology Department; Brown University, Department of Ecology and Evolutionary Biology;

Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.

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