Construction Of Synthetic Phage Displayed Fab Library-Preview

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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity - a 2 minute Preview of the Experimental Protocol

Ganggang Huang, Zhenwei Zhong, Shane Miersch, Sachdev S. Sidhu, Shin-chen Hou, Donghui Wu
ShanghaiTech University, Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies; University of Toronto, Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research; University of Toronto, Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research;

This protocol describes a detailed procedure for the construction of a phage-displayed synthetic antibody library with tailored diversity. Synthetic antibodies have broad applications from basic research to disease diagnostics and therapeutics.

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