SNEV Characterization Series: EV analysis by flow cytometry

Estefanía Lozano Andrés: EV analysis by flow cytometry: Navigating challenges and unleashing opportunities

The investigation of EVs is complicated due to their small size, heterogeneity and complexity, which has resulted in a plethora of detection methods. Consequently, efforts have been initiated to establish guidelines, reference materials and reporting frameworks that will fuel robustness in EV research. At the same time, the EV community is looking for technological solutions for the detection of single EVs. Such technologies would ideally be high-throughput while providing quantitative and qualitative information. Flow cytometry was originally designed for cells, whose signals are well above the detection limit of instruments, in sharp contrast to the dim signals of EVs. Still, flow cytometry is a promising technology and currently one of the methods of choice for EV characterization. EVs, however, are orders of magnitude smaller than cells and their signals are often below or close to the detection limit of the instruments, which causes a number of limitations and challenges that need to be accounted for. In this talk we will cover the basics of EV flow cytometry while addressing advanced tools for standardization and show illustrative examples of how flow cytometry can help us unravel the complexity of single EV-based analysis in biofluids.

This talk is essential viewing for anyone interested in the future of EV biology and therapy. If you are a researcher, engineer, or student working in the field of EVs, then you will not want to miss this opportunity to learn about the latest innovations in EV characterization.

This event happened on March 2024

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