Luminex MultiCode®-RTx Technology

What is Luminex® MultiCode®-RTx Technology, and how does it work?

MultiCode-RTx Technology is a novel chemistry for nucleic acid-based assays and is used for our probe free, real-time PCR multiplex assay. Products using the technology offer a flexible platform for both real-time and multiplex PCR-based assays.

MultiCode uses isomers of the base pair Cytosine and Guanine, or Iso C and Iso G. The synthetic isoC:isoG DNA base pair differs from the naturally occurring base pair in its hydrogen bonding pattern. As a result, the MultiCode bases, isoC and isoG, can only base pair with each other. This property enables site-specific incorporation of the isobases during amplification. By using reporter tag to isoC, and quencher tag to isoG base pairs, a decrease in fluorescence can be measured when they combine.

https://www.luminexcorp.com/multicode...