LC Troubleshooting—All of My Peaks are Tailing! What Should I Do?

Tailing peaks can be a problem when we are doing liquid chromatography (LC), and secondary silanol interactions are one of the causes. Here, using the example of basic drugs, we explain what causes them and cover a few steps that we can take to minimize the effect of these secondary silanols on our peak shape when we are troubleshooting.

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Additional Resources

All of My Peaks are Tailing! What Should I Do? | https://www.restek.com/chromablograph...

Troubleshooting HPLC – Tailing Peaks | https://www.restek.com/chromablograph...

When Should You Use a Buffer for HPLC? | https://www.restek.com/chromablograph...

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Restek Recommended Tools & Parts

Mobile Phase Accessories | https://www.restek.com/c/1123

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