What Do You Do with a New LC Column?

Before you can use a new LC column to perform analyses, there are a few important steps you need to do to prepare it for its new life in your lab.

In this Restek Tip, we show you how to set up your new LC column. We cover certificate of analyses (CofAs), discuss the different column end-fittings you can use, share how to condition your column for your mobile phase, and finally, we’ll look at how to select an appropriate flow rate for your column. Let’s get started!

-----------------------------------------------------------------

Contents

0:00 Preparing a New LC Column

0:46 LC Certificates of Analysis

1:20 Connecting Your LC Column

3:50 Conditioning Your LC Column

4:46 Selecting an Appropriate Flow Rate

-----------------------------------------------------------------

Additional Resources

Restek Documentation Search | https://www.restek.com/documentation-...

Solvent Miscibility and Solubility Chart | https://www.restek.com/articles/solve...

Restek LC Column Use and Care Instruction Sheet | https://www.restek.com/articles/reste...

HPLC and UHPLC FAQ | https://www.restek.com/articles/frequ...

-----------------------------------------------------------------

Restek Recommended Tools & Parts

EXP Reusable Fittings | https://www.restek.com/p/25937

Restek’s HPLC & UHPLC Columns | https://www.restek.com/c/1119

Restek’s LC Accessories
- LC-MS Supplies | https://www.restek.com/c/1122
- LC Autosampler Supplies | https://www.restek.com/c/1118
- Mobile Phase Management | https://www.restek.com/c/1123
- LC Detector Supplies | https://www.restek.com/c/1120
- LC Tubing & Fittings | https://www.restek.com/c/1121
- LC Pump Supplies | https://www.restek.com/c/1124
- Tools & Organization | https://www.restek.com/c/1125

-----------------------------------------------------------------

Connect with Us

Comment on this video and subscribe to Restek's YouTube channel today at    / restekcorp  

Website: https://www.restek.com

LinkedIn:   / restek-corporation  

Facebook:   / restekcorporation