CHEMetrics I-2017 Detergents Test Demonstration

RINSE WITH SAMPLE & FILL TO 15 ML
Rinse the red-tipped plastic dropper bottle with the sample to be tested, then fill it to the 15 mL fill line.

SNAP FIRST TIP
While holding the double-tipped ampoule in a vertical position, snap the upper tip using the tip breaking tool. Invert the ampoule and position the open end over the dropper bottle.

SNAP SECOND TIP
Snap the upper tip and allow the contents to drain into the dropper bottle.

CAP AND SHAKE 30 SECS
Cap the dropper bottle and shake it vigorously for 30 seconds. While shaking the bottle, apply pressure to the red cap with your thumb to ensure that no leaking occurs.

WAIT 1 MINUTE
Allow the dropper bottle to stand upright and undisturbed for 1 minute. The layers should separate in the dropper bottle. During this period, gently loosen the screw cap to release the pressure created by the shaking, then re-tighten the cap.

REMOVE CAP & INVERT
Remove the red cap from the dropper bottle, then slowly invert the dropper bottle over a test tube and squeeze the bottle to deliver only the chloroform layer into the test tube.

WAIT 4 MINUTES
Allow the test tube to stand upright and undisturbed for 4 minutes.

WIPE & INSERT TUBE INTO SAM PHOTOMETER AFTER ZEROING TO OBTAIN READING

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