4) CRISPR Cas9 - Gene Regulation with dCas9

The CRISPR Cas9 system has revolutionized the field of genetic engineering with its simplicity and versatility. Now, scientists have cleverly created a catalytically dead Cas9 (dCas9) that is able to bind its target with high specificity without cutting any DNA. This new CRISPR Cas9 system expands the field a step further as a novel new tool for reversible gene regulation. As you will learn in this video, dCas9 can act as a modular platform upon which activators or repressors can be easily fused and targeted to the gene of interest.

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