Microfluidic generation of double emulsions for fluorescence-activated cell sorting (FACS)

Screening of individual cells for an enzymatic activity or antibodies using fluorescence-activated cell sorting (FACS) is an efficient approach for library screening. An example of an application that can use FACS sorting of libraries is directed evolution for protein engineering, which can be very useful in activities such as improving antibodies and other therapeutic proteins.

Microfluidics allows screening on a single cell level, at high throughput, and most importantly for genotype-phenotype coupling. The small (picolitre) droplet volume means this method reduces assay volume which ultimately leads to cost savings. The droplets compartmentalise individual cells entrapping all secreted molecules and analytes, preventing interference with other cells and products.

This webinar hosted by Dr Gurinder Vinner will introduce you Dolomite’s methods of double (water-in-oil-in-water) emulsion production for FACS using our uEncapsulator System.

1) Introduction: 0:00 - 1:51
2) Introduction of the Particle Engineering Team: 1:51 - 3:05
3) Why do we use microfluidics? 3:06 - 4:42
4) Use of double emulsions for FACS 4:43 - 8:00
5) Introduction to the uEncapsulator System 8:01 - 9:35
5.1) System components 9:36 - 11:42
6) uEncapsulator double emulsions 11:42 - 14:50
7) Practical system demonstration 14:50 - 19:26
7.1) Software demonstration for primary emulsion step 19:26 - 22:07
7.2) Double emulsion step 22:07 - 22:48
7.3) Software demonstration for double emulsions 22:48 - 24:36
8) FACS sorting 24:36 - 27:45
9) Summary 27:45 - 29:34

To find out more please do not hesitate to contact us at: [email protected]
Or visit our website: dolomite-microfluidics.com