Скачать или смотреть You can improve the linearity & sensitivity of a Bradford assay by taking 595(or 590)/450 ratio

Doing this takes into account the fact that the amount of free dye (which measures most strongly around 450, but bleeds into the 590 range where the dye-bound form peaks) is decreasing, making your background decrease and disfavoring binding as the protein concentration increases.

Zor, T.; Selinger, Z. Linearization of the Bradford Protein Assay Increases Its Sensitivity: Theoretical and Experimental Studies. Analytical Biochemistry 1996, 236 (2), 302–308. https://doi.org/10.1006/abio.1996.0171.

Ernst, O.; Zor, T. Linearization of the Bradford Protein Assay. J Vis Exp 2010, No. 38, 1918. https://doi.org/10.3791/1918.

More on the protein quantification assays:: http://bit.ly/proteinmeasuring &    • Overview of protein concentration determin...  

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