Скачать или смотреть Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen!

Imidazole is great for purifying His-tagged proteins, but it's a pain for when you want to detect and measure concentration of proteins using UV280 absorbance!

More on His-tag purification: http://bit.ly/histagpurification

More on determination of protein concentration: http://bit.ly/proteinmeasuring

More on chromatograms: https://bit.ly/chromatograms

More on elution types: https://bit.ly/elution &    • Elution terms, strategies (stepwise, gradi...  

More on desalting & buffer exchange: https://thebumblingbiochemist.com/365... &    • Techniques for buffer exchange (Desalting ...  

more on protein chromatography: http://bit.ly/proteincleaning &    • Protein chromatography  

more practical lab tips & tricks: https://bit.ly/lab_tricks_page &    • Practical lab tips & tricks        
    
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com        

#scicomm #biochemistry #molecularbiology #biology #sciencelife #science #realtimechem