How to measure the pH of culture media - Microbiology Lab Tutorial

In this video tutorial, we will demonstrate the process of using a pH meter to measure the pH of culture media.

When culturing microbes, it is critical that the media used meets the growth requirements of the target organism. For this reason, it is necessary to measure the pH of culture media during preparation to ensure that the media will perform as intended.

Most microbes grow best at a neutral or slightly alkaline pH. For this reason, it's no coincidence that the common general growth medium Tryptic Soy Agar is prepared with a pH specification of 7.1 to 7.5.

00:00 Intro
00:09 Info about the pH scale
01:01 History of pH
01:16 Before you get started
01:40 Calibrating your pH meter
03:10 Verifying your pH meter readings
05:01 Reading pH of culture media
05:39 Closing info

In addition to culture media, Hardy Diagnostics offers lab supplies like pH meter calibration buffers:
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