How to prepare the perfect Gram stain - Gram staining procedure

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In this educational video from Hardy Diagnostics, we detail what a Gram stain is, what is the purpose of a Gram stain procedure, and share how you can perform a perfect Gram stain test.

A written step-by-step version of this tutorial can be found on our blog: https://hardydiagnostics.com/blog/gra...

Q-Slide Gram (Cat. no. Z302)
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Gram Stain Kit Advanced™ (Cat. no. GK400A)
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00:00 History of the Gram stain
00:20 The importance of the Gram stain
00:38 Types of bacteria
00:53 The cell wall
01:27 Beginning the Gram stain
02:09 Fixing the slide with methanol
02:53 Crystal violet
03:02 Deionized water
03:12 Gram's iodine
03:26 Rinse with deionized water
03:37 Decolorizer
04:58 Color of Gram positive vs Gram negative
05:11 Counterstain with safranin
05:26 Rinse and dry
05:50 Summary
06:13 How to view slide under a microscope
06:30 Results
06:47 Ordering info and close

Check out our other laboratory tutorials on our blog:
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Filmed and Edited by Brian Tom

#Microbiology #GramStain #Bacteria #HardyDiagnostics #gramstaining