Droplet sample preparation for single-cell proteomics | Andrew Leduc | SCP2021

Presentation by Andrew Leduc at the 4th single-cell proteomics conference, SCP2021: https://single-cell.net/proteomics/sc.... Droplet sample preparation for single-cell proteomics. See more at: https://sample-prep.slavovlab.net & http://andrewdleduc.com.

For more details, see:
Leduc A, Huffman RG, Slavov N. (2021)
Droplet sample preparation for single-cell proteomics applied to the cell cycle
bioRxiv, doi: 10.1101/2021.04.24.441211, https://doi.org/10.1101/2021.04.24.44...

Droplet sample preparation websites: https://scp.slavovlab.net/nPOP and https://sample-prep.slavovlab.net/

Mass spectrometry methods have enabled quantifying thousands of proteins at the single cell level. These methods open the door to many biological opportunities, such as characterizing heterogeneity in the tumor micro-environment, signaling pathways driving stem cell differentiation, and intrinsically single-cell processes, such as the cell division cycle. To further advance single-cell MS analysis, we developed an automated nano-ProteOmic sample Preparation (nPOP). nPOP uses piezo acoustic dispensing to isolate individual cells in 300 picoliter volumes and performs all subsequent preparation steps in small droplets on a fluorocarbon slide. This allows massively parallel sample preparation, including lysing, digesting, and labeling individual cells in volumes below 20 nanoliters.