LC Troubleshooting—Optimizing Your Injection to Improve Peak Shape

Injection volume and solvent choice are among the many factors that contribute to good liquid chromatography. If we inject too much, we can drastically degrade peak shape by causing our peaks to front. At the same time, we want to choose an injection solvent that matches our initial mobile phase conditions.

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Additional Resources

HPLC and UHPLC Frequently Asked Questions | https://www.restek.com/articles/frequ...

Solvent Mismatch Effects on Peak Shape for LC Analysis | https://www.restek.com/chromablograph...

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Restek Recommended Tools & Parts

LC Syringes | https://www.restek.com/search/syringe...

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