Edman degradation | Edman Sequencing

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Edman degradation is a a method used for the sequencing of peptide/protein. In this method the peptide is allowed to react with edmans reagent under mild alkaline conditions. The edmans reagent reacts with
the N terminal amino acid and forms a complex. This complex is then cleaved from the peptide and separated by chromatography or electrophoresis. The method can be repeated several times to find the sequence of the peptide.

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