Edman Degradation: A Step-by-Step Guide || How to Sequence Proteins Using Edman Degradation

Описание к видео Edman Degradation: A Step-by-Step Guide || How to Sequence Proteins Using Edman Degradation

This video is related to Edman degradation.
Edman degradation is a method for sequencing proteins. In this video, we'll walk through the Edman degradation reaction step-by-step. We'll start by learning the basics of the reaction, and then we'll see how it's used to sequence proteins.

We'll also discuss the advantages and disadvantages of Edman degradation, and we'll look at some of the applications of this technique. By the end of this video, you'll have a good understanding of how Edman degradation works and how it can be used to sequence proteins.
Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues.
The Edman method is an excellent and widely adopted method for determining the amino acid sequence in proteins and peptides. The method identifies amino acid residues one by one from the N-terminal side.
Since the automated Edman method was developed by Edman and Begg, microanalysis and acceleration have been required, and analyses in the order of pmol have been performed using a sequencer(PPSQ).
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