Running qPCR of cDNA

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This tutorial video is a follow up of the RNA isolation video. Here I show the qPCR set up and process. I used mouse retinal micro-endothelial cells (MRMEC) cDNA and primers from TaqMan system.

[Time stamps]
00:00 - Intro
01:00 - cDNA dilution calculations
02:02 - Diluting cDNA
06:51 - qPCR Protocol Overview
11:32 - Introducing QuantStudio3 System
13:40 - Plate set up in the QuantStudio3 software
20:26 - Preparing TaqMan mix with primers and water
24:47 - Loading samples onto 96-well plate
33:02 - Running qPCR
35:10 - Finish qPCR run and storing Data