cDNA synthesis Tutorial

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The cDNA synthesis tutorial video is the follow up step after isolating RNA. We are able to synthesize cDNA from RNA through a process called reverse transcription (RT). RT is a process that does not occur in mammalian cells and it is not part of the Central Dogma in Biology. In order to perform RT we make use of a retrovirus enzyme called reverse transcriptase, which is able to synthesize cDNA from RNA.

[Time stamps]
0:00 - introduction
3:43 - components
5:17 - measuring RNA concentration
6:26 - loading samples onto Take3 Trio plate
8:27 - BioTek Cytation5 software
12:00 - Calculations to run cDNA synthesis
16:33 - Mixing all components for RT
28:22 - Running RT using thermocycler

Links to other tutorial videos associated with this video
1) Isolating RNA from primary cells
   • Isolating RNA from primary cells  
2) Running qPCR of cDNA
   • Running qPCR of cDNA