Скачать или смотреть Myosin Spin Down Protocol

Daisy Achiriloaie demonstrates how to spin down reconstituted myosin II motor proteins. Dead myosin in the reconstituted sample cannot unbind from filamentous actin, so centrifugation causes the dead myosin proteins to form a pellet with the much heavier actin, allowing the active myosin II to be extracted as the supernatant.


Filming and production by Nathan Fletcher (Sheung Lab).
Youtube uploads by Gregor Leech (Anderson/ McGorty Lab).

Special thanks to Principal Investigators Dr. Janet Sheung, Dr. Ryan McGorty, and Dr. Rae Robertson-Anderson for providing equipment and support for this video.

Check out our lab websites for more research and information!
Robertson-Anderson Lab → http://www.biospotlab.com/
McGorty Lab → https://rmcgorty.github.io/
Sheung Lab → https://sites.google.com/view/sheunglab/home