Скачать или смотреть How to Flash Freeze With Myosin Reconstitution

Daisy Achiriloaie explains how to reconstitute powdered myosin II motor proteins in DTT, and how to flash freeze the reconstituted myosin in liquid nitrogen. Flashing freezing proteins allows for long-term storage of aliquots in a -80C freezer.


Filming and production by Nathan Fletcher (Sheung Lab).
Youtube uploads by Gregor Leech (Anderson/ McGorty Lab).

Special thanks to Principal Investigators Dr. Janet Sheung, Dr. Ryan McGorty, and Dr. Rae Robertson-Anderson for providing equipment and support for this video.

Check out our lab websites for more research and information!
Robertson-Anderson Lab → http://www.biospotlab.com/
McGorty Lab → https://rmcgorty.github.io/
Sheung Lab → https://sites.google.com/view/sheunglab/home