How to identify Staphylococcus aureus using the Coagulase Test - Slide and Tube Method

The coagulase test is commonly used by microbiologists to assist in the rapid identification of Staphylococcus aureus. Coagulase is a bacterial enzyme that converts fibrinogen to fibrin, resulting in blood clotting. Because bacteria such as Staphylococcus aureus have coagulase bound to their surface, they can disguise themselves as a blood clot, thus preventing phagocytosis by white blood cells.

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CoaguStaph™ Rabbit Coagulase Plasma with EDTA, Freeze Dried, for Staphylococcus aureus, 6x3ml vials Cat. no. Z003
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CoaguStaph™ Rabbit Coagulase Plasma with EDTA, Freeze Dried, for Staphylococcus aureus, 6x20ml vials Cat. no. Z020
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Coagulase Cryo (Ready to Use Coagulase Plasma), 0.5ml, Cryovial, 20 tubes Cat. no. Z202
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00:00 History of coagulase test
00:28 Types of coagulase
00:38 Bound and free coagulase
00:49 CoaguStaph™
00:58 More info about coagulase
01:24 False negatives
01:58 What you will need for the slide test
02:14 Starting the coagulase slide test
02:59 Observations
03:27 Tube coagulase test
03:39 What you need for the tube test
03:56 Start of tube coagulase test
04:24 Storing CoaguStaph™
04:32 Inoculating the CoaguStaph™
05:09 Incubating the tubes
05:22 Interpreting the results
05:49 Closing info

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