Important Notices for Daily Use of HPLC: 2. Abnormal peak shape (Effect of column degradation)

These are the most likely causes for abnormal peak shape.
e.g.: Column degradation/ Effect of sample solvent/ Effect of mobile phase composition/ Temperature distribution in analytical column/ Effect of filtration setting in detector
We talk about countermeasures for these while showing respective examples.

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00:00 start
00:12 What is abnormal peak shape?
00:34 Column degradation
01:23 Important notices for long term use
02:00 Confirming column performance
03:40 Protection of analytical column
05:17 Column rinsing

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