Composition and prepration of 50x TAE Buffer stock (Tris acetate EDTA)

Описание к видео Composition and prepration of 50x TAE Buffer stock (Tris acetate EDTA)

TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. In molecular biology it is used in agarose electrophoresis typically for the seperation of nucleic acids such as DNA and RNA. It is made up acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. Here we have made 50x TAE buffer and mentioned a step wise protocol for prepration of 1 litre stock, for 2 litre you have to double the measurments and quantity of chemicls used. You can use it for electrophoresis with 10x and 1x measures according to your requirements. For more details or related quries, comment down below and make sure to subscribe ‪@ampliconsofbiotech‬

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