Lambda Phage and Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

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This video discusses the general biology of lambda bacteriophage and cosmid cloning vectors. There are two types - insertional and replacement - of vectors derived from lambda bacteriophage. This requires the use of spi phenotype since bacteriophage vectors have no selection or screening markers. From the bacteriophage vector, cosmids are created, relying on the unique function of cos sites. Understanding cosmid means understanding the structure and function of cos sites. Cosmid containing viruses are assembled in vitro by extracting virus proteins from bacteria and mixing it with the cosmid DNA in a tube.

Jump to your favorite section:
00:00 Lambda vector
02:12 Insertional vector
03:02 Replacement vector
04:30 spi phenotype
06:27 Cos site
08:27 Cosmids
10:58 In vitro packaging
12:14 Cosmid Concatemers
14:38 Phosmid

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