Brain Microvascular Endothelial Cells Isolation | Protocol Preview

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Isolation of Primary Murine Brain Microvascular Endothelial Cells - a 2 minute Preview of the Experimental Protocol

Tobias Ruck, Stefan Bittner, Lisa Epping, Alexander M. Herrmann, Sven G. Meuth
University of Münster, Department of Neurology; Interdisciplinary Center for Clinical Research (IZKF) Münster,; University of Münster, Institute of Physiology I — Neuropathophysiology I;

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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