SDS PAGE

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SDS PAGE (Polyacrylamide gel electrophoresis) is a common lab technique used for the separation and detection of proteins. SDS PAGE uses polyacrylamide gel that is formed by the polymerization of acrylamide and cross linking agent bis-acrylamide in presence of APS and TEMED. Native Polyacrylamide gel electrophoresis (native PAGE) separates proteins based on the size as well as net electric charge on the protein. Where as in SDS Polyacrylamide gel electrophoresis (SDS PAGE) the proteins attain the net negative charge and the separation occurs based on the size of proteins.

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