Explant Culture of Allantois to analyse Allantoic Attachment | Protocol Preview

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Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment - a 2 minute Preview of the Experimental Protocol

Kerstin Hadamek, Angelika Keller, Antje Gohla
University of Würzburg, Institute of Pharmacology and Toxicology; University of Würzburg, Rudolf Virchow Center for Experimental Biomedicine;

We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.

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