Micrometastases Detection generated by Organoids | Protocol Preview

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High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor - a 2 minute Preview of the Experimental Protocol

Yu Okazawa, Kosuke Mizukoshi, Yu Koyama, Shoki Okubo, Hiromitsu Komiyama, Yutaka Kojima, Michitoshi Goto, Sonoko Habu, Okio Hino, Kazuhiro Sakamoto, Akira Orimo
Juntendo University Faculty of Medicine, Department of Coloproctological Surgery; Juntendo University Faculty of Medicine, Department of Molecular Pathogenesis; Juntendo University Faculty of Medicine, Atopy Research Center; Tokyo Dental College, Department of Oral Pathobiological Science and Surgery; Juntendo University Faculty of Medicine, Department of Gastroenterology;

To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.

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